| 1000043081 | Officina REV srl | IT-Italy | Company | No | Incubatee | ESA BIC Turin | |
| 1000046769 | Oinride Oy | FI-Finland | Company | No | Incubatee | ESA BIC Finland | |
| 1000031421 | OKAPI:Orbits GmbH | DE-Germany | Company | Yes | Alumnus | ESA BIC Hessen | |
| 1000042494 | Okeanos Smart Data Solutions GmbH | DE-Germany | Company | No | Incubatee | ESA BIC North Rhine-Westphalia | |
| 1000042424 | Olive Robotics GmbH | DE-Germany | Company | No | Alumnus | ESA BIC Bavaria | |
| 1000030044 | OMANOS ANALYTICS | GB-United Kingdom | Company | Yes | Alumnus | ESA BIC UK | |
| 1000032989 | Ommatidia Lidar S.L. | ES-Spain | Company | Yes | Alumnus | ESA BIC Madrid Region | |
| 1000043603 | onocoy Services AG | CH-Switzerland | Company | No | Incubatee | ESA BIC Switzerland | |
| 1000027054 | OPT ALM | FR-France | Company | Yes | Alumnus | ESA BIC Sud France | |
| 1000030217 | OPT/NET BV | NL-Netherlands | Company | Yes | Alumnus | ESA BIC Noordwijk | |
| 1000044838 | Optocycle GmbH | DE-Germany | Company | No | Alumnus | ESA BIC Baden-Württemberg | |
| 1000029654 | Orbital Astronautics | GB-United Kingdom | Company | Yes | Alumnus | ESA BIC UK | |
| 1000030848 | ORBITAL CARGO DRONE LTD | GB-United Kingdom | Company | No | Alumnus | ESA BIC UK | |
| 1000013313 | ORBITAL EYE BV | NL-Netherlands | Company | No | Alumnus | ESA BIC Noordwijk | |
| 1000042990 | Orbital Matter Poland Sp. z o.o. | PL-Poland | Company | Yes | Alumnus | ESA BIC Poland | |
| 1000040177 | Orbital Outpost Italy S.R.L. | IT-Italy | Company | No | Alumnus | ESA BIC Turin | |
| 1000043214 | Orbital Paradigm | ES-Spain | Company | Yes | Incubatee | ESA BIC Madrid Region | |
| 1000029399 | Orbital Witness Limited | GB-United Kingdom | Company | No | Alumnus | ESA BIC UK | |
| 1000044988 | Oris | IT-Italy | Company | No | Incubatee | ESA BIC Turin | |
| 1000031454 | OroraTech GmbH | DE-Germany | Company | Yes | Alumnus | ESA BIC Bavaria | |